Regeneration of
pH glass electrodes

The pH glass membrane, the probe tip, is the sensitive part of your electrode. If the glass membrane dries out during storage and sometimes after a rough cleaning procedure gets damaged, you need to restore the hydrated glass layer.

Below are suggestions for three different regeneration methods you can use. The first method is the easiest to handle and the third method is the most powerful.

What method you choose to use will depend on your experience and knowledge about the pH electrodes you are using. If it's the first time you regenerate your pH glass electrode, start with the first method. If this not helps and the electrode behaves in an inappropriate way, as a slow and sluggish response, take the next step and try method 2. You still have a problem with the pH electrode? Okay, it's time to try the "final step", method 3.

Regeneration method 1

Regeneration method 2

  • Soak the pH electrode overnight in 0.1 M HCl.
  • Rinse the electrode carefully in water.
  • Soak the electrode in buffer 4 for about one hour.
  • Rinse.

Regeneration method 3

Manufacturers of pH electrodes usually sell their special solution for regeneration of glass electrodes. An example of a regeneration solution is a mixture of hydrochloric acid (HCl) and hydrofluoric acid (HF). For the appropriate procedure, see the instruction manual for the regeneration solution.

However, general guidelines for using a reactivation solution are:

  • Immerse only the tip of the electrode, the pH sensitive glass membrane, in a sample of regeneration solution for 5 to 15 minutes. It is important to avoid any contact with the electrode body and the regeneration solution. The electrode body can be damaged by the aggressive solution.
  • Rinse carefully and thoroughly with water for 1-3 minutes.
  • Soak the pH electrode in buffer 7 for about one hour.
  • Allow the electrode to stand in reference electrolyte solution, overnight (see method 1).

Be careful when handling a reactivation solution. It is very toxic and aggressive to clothing, skin, and especially eyes.

Ready to be used

If your regeneration procedure has been successful, your electrode is now ready for calibration and measurement.

Share your point of view with others!

Take the opportunity to share your knowledge and experiences with others.

Please, use the form below to publish your contribution about the subject "Regeneration of pH glass electrodes". Before your page is published, it will be reviewed to make sure it applies to the submission guidelines for this website.

Don't forget to read what others have written, maybe you have a comment to add.

What Other Visitors Have Said

Click below to see contributions from other visitors to this page...

Regeneration procedure 
The regeneration procedure varies from sensor to sensor. Check the manufactures specifications and recommended procedures. Never condition the pH glass …

Click here to write your own. - Click Here for ALL Your Office Supply Needs!

ph glass

Favorite pages:

acids and bases
What is an acid? What is a base? If you want to know, read here!

calculating ph
Do you know what pH stand for? If not, click here!

electrode design
The pH electrode consists of the following parts:
. . read here!
offset error
Real-world pH electrodes are not ideal,
read why.

definition of ph
What is the definition of pH? See the answer. . .

nonglass ph electrode
Is it not possible to use a glass electrode for your application? Use a non-glass pH electrode.

ph measurement
Learn how the temperature affects your pH measurement.

Download the new release ISO 14001:2015